Mouse Monoclonal IgG2a + IgG1 Basal Cell Marker Cocktail 1, p63 Protein + HMW Cytokeratin

• Catalog number
  BAS001-0.5
• Price:

  Please ask

  Ask for price
• Size
  0.5 ml

• Availability
  Please contact us to check the availability
• Immunogen
  Recombinant human p63 protein (aa 1-205) and cytokeratin extract of human stratum corneum
• Specificity
  nuclear p63 and cytoplasmic high molecular weight cytokeratins
• Species reactivity
  Human
• Product presentation
  Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for 125-250 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
• Product category
  Primary Antibodies
• Antibody s raised in
  Mouse
• Antibody s isotype
  IgG2a + IgG1
• Antibody s clone
  4A4 + 34ßE12
• Product is supplied as
  liquid concentrate
• Recommended concentration for use
  1:25-1:50
• Protein concentration
  See the included datasheet
• Positive control
  Normal prostate tissue or skin
• Pretreatment
  Use formalin-fixed and paraffin-embedded sections; Retrieval conditions: Unmasking fluid T, TEC buffer (Tris/EDTA/Citrate) pH 8 (Art. No. DE005) in a pressure cooker at 100°C 20-40 minutes
• Tested applications
  IHC(P)
• Recommended secondary reagent
  We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005), but also other staining systems including anti-mouse IgG will be suitable
• Storage method
  Store at 2-8°C
• UniProt number
  Go to UniProt/SwissProt
• Litterature
  1. Signoretti S., Waltregny D., Dilks J., Isaak B., Lin D., Garraway L., et al. (2000) p63 is a prostate basal cell marker and is required for prostate development. Am J. Pathol. 157; 1769-1775. 2. Weinstein M.H., Signoretti S., and Loda M. (2002) Diagnostic utility of immunohistochemical staining for p63, a sensitive marker of prostatic basal cells. Mod. Pathol. 15(12); 1302-1308 3. Yang X.J., Lecksell K., Gaudin P., and Epstein J.I. (1999) Rare expression of high-molekular-weight cytokeratin in adenocarcinoma of the prostate gland: a study of 100 cases of metastatic and locally advanced prostate cancer. Am. J. Surg. Pathol. 23(2); 147-152. 4. Browne TJ, Hirsch MS, Brodsky G, Welch WR, Loda MF, Rubin MA. Prospective evaluation of AMACR (P504S) and basal cell markers in the assessment of routine prostate needle biopsy specimens. Hum Pathol. 2004 Dec;35(12):1462-8. 5. Jiang Z, Li C, Fischer A, Dresser K, Woda BA. Using an AMACR (P504S)/34betaE12/p63 cocktail for the detection of small focal prostate carcinoma in needle biopsy specimens. Am J Clin Pathol. 2005 Feb;123(2):231-6.
• Tips
  *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
• Other products
• Description
  For cells, cell lines and tissues in culture till half confluency.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Monoclonal   IgG2a   IgG1   Basal   Cocktail   p63   Protein   HMW   Cytokeratin  
• Gene symbol
  CFAP97, CSPG4
• Short name
  Bioprime
• Technique
  Mouse monoclonal, Mouse, Component cell markers and DNA bp. markers are genetic markers er genes or DNA sequences with a known location on a chromosome that can be used to identify individuals or species. Protein markers in kDA. Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
• Host
  mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
• Isotype
  IgG1, IgG2a
• Species
  Mouse, Mouses
• Alternative name
  Basal Cell Marker Cocktail 1, p63 Protein + HMW Cytokeratin
• Alternative technique
  murine
• Tissue
  cell

Gene info

• Identity
  HGNC:29276
• Gene
  CFAP97
• Long gene name
  cilia and flagella associated protein 97
• Synonyms gene
  • KIAA1430
• Synonyms gene name
  • KIAA1430
• Synonyms
  • DKFZp434F1728
  • hmw
• GenBank acession
  • AB037851
• Locus
  4q35.1
• Discovery year
  2004-04-21
• Entrez gene record
  57587
• Pubmed identfication
  • 10718198
  • 24554765
  • 26638075
• RefSeq identity
  • NM_020827
• Classification
  • Cilia and flagella associated
• VEGA ID
  OTTHUMG00000160468

Gene info

• Identity
  HGNC:2466
• Gene
  CSPG4
• Long gene name
  chondroitin sulfate proteoglycan 4
• Synonyms gene name
  • chondroitin sulfate proteoglycan 4 (melanoma-associated)
• Synonyms
  • MCSPG
  • MEL-CSPG
  • MSK16
  • NG2
  • MCSP
  • HMW-MAA
  • CSPG4A
• Synonyms name
  • melanoma-associated chondroitin sulfate proteoglycan
• GenBank acession
  • X96753
  • AY359468
• Locus
  15q24.2
• Discovery year
  1997-11-28
• Entrez gene record
  1464
• Pubmed identfication
  • 8790396
  • 16407841
• RefSeq identity
  • NM_001897
• Classification
  • Proteoglycans
• VEGA ID
  OTTHUMG00000142836

Product images

Similar products