Mouse IL-2 PicoKine ELISA Kit

#

• Catalog number
  EK0398
• Price:

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• Size
  1x96 well plate

# #

• ELISA s application
  For quantitative measurement of mouse IL-2 in cell culture supernatant, serum and plasma heparin, EDTA, citrate .
• Gene tested
  murine IL-2
• ELISA standard curve
  Concentration(pg/ml) + 0 + 15.6 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.067 + 0.111 + 0.166 + 0.288 + 0.453 + 1.080 + 1.669 + 2.221
• ELISA kit detection limits
  15.6pg/ml to 1000pg/ml
• ELISA s sensitivity
  < 1pg/ml
• Target of this ELISA
  Natural and recombinant mouse IL-2
• Cross reaction
  the ELISA does not cross react with any other proteins except from closely related species
• ELISA s precision parameters
  intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);81;215;590;86;193;575 ELISA standard deviations 4.536;11.61;25.37;5.246;13.32;29.9 CV(%);5.6;5.4;4.3;6.1;6.9;5.2
• ELISA kit description
  GENTAUR's mouse IL-2 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for IL-2 has been precoated onto 96-well plates. Standards (E.coli,A21-Q169) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-2 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse IL-2 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
• Detector and capure antibodies
  A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
• The ELISA contains
  Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse IL-2 antibodies;1 ST0000-10;Lyophilized recombinant mouse IL-2 standard;10ng/tubex2 AR1107;Biotinylated anti- mouse IL-2 antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
• Anti coagulation
  heparin, EDTA or citrate
• How the elisa works
  Sample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml mouse IL-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma( heparin, EDTA, citrate) to the ELISA plate. Keep in mind that each mouse IL-2 standard solution and each sample be measured in duplicate.
• Minutes of TMB incubation
  25-30
• More gene info
  Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4. Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.
• Antigen
  E.coli,A21-Q169
• Keep at
  Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
• Uniprot
  Interleukin-2
• Gene name
  interleukin 2
• Alternative
  Aldesleukin;IL 2;IL-2;IL2;IL2_HUMAN;Interleukin 2;Interleukin-2;Interleukin2;Involved in regulation of T cell clonal expansion;Lymphokine;T cell growth factor;T-cell growth factor;TCGF
• Gen protein identity
  P04351
• Entrez nr
  16183
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

#

• Gene target
  IL-2   PicoKine  
• Gene symbol
  IL2
• Short name
  Mouse IL-2 PicoKine ELISA Kit
• Technique
  ELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
• Host
  mouse, antigen assay ELISA kit 1 Kit (96 Wells)
• Species
  Mouse, Mouses
• Alternative name
  Mouse Interleukin-2 PicoKine Enzyme-linked immunosorbent assay reagent
• Alternative technique
  murine, elisas, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:6001
• Gene
  IL2
• Long gene name
  interleukin 2
• Synonyms
  • IL-2
  • TCGF
• Synonyms name
  • T cell growth factor
• GenBank acession
  • U25676
• Locus
  4q27
• Discovery year
  1986-01-01
• Entrez gene record
  3558
• Pubmed identfication
  • 3260003
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000133075

MeSH Data

• Name
  Enzyme-Linked Immunosorbent Assay
• Concept
  Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
• Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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