KOMBITEST CD3 FITC / CD4 PE / CD45 PerCP

Description

CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
Properties

This Exbio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exbio FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody
Conjugate

Peridinin chlorophyll (PerCP)

Gene target

KOMBITEST CD3 CD4 CD45
Gene symbol

CD4, PTPRCAP, PTPRC
Short name

KOMBITEST CD3 FITC / CD4 PE / CD45 PerCP
Technique

Percp, FITC, Fluorescein, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the KOMBITEST CD3 FITC / CD4 PE / CD45 PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from Exbio.
Label

FITC
Alternative name

KOMBITEST CD3 fluorecein / CD4 molecule peroxidase / CD45 PerCP
Alternative technique

antibodies-percp, fluorescine
Alternative to gene target

CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098

Identity

HGNC:1678
Gene

CD4
Long gene name

CD4 molecule
Synonyms gene name
- CD4 antigen (p55)
- T-cell surface glycoprotein CD4
GenBank acession
- M35160
Locus

12p13.31
Discovery year

1986-01-01
Entrez gene record

920
RefSeq identity
- NM_000616
Classification
- CD molecules
- C2-set domain containing
- V-set domain containing
VEGA ID

OTTHUMG00000168514

Identity

HGNC:9666
Gene

PTPRC
Long gene name

protein tyrosine phosphatase receptor type C
Synonyms gene
- CD45
Synonyms
- LCA
- T200
- GP180
GenBank acession
- Y00062
Locus

1q31.3-q32.1
Discovery year

1986-01-01
Entrez gene record

5788
Pubmed identfication
- 2169617
RefSeq identity
- NM_002838
Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
- CD molecules
VEGA ID

OTTHUMG00000035702
Locus Specific Databases
- PTPRCbase: Mutation registry for CD45 deficiency
- LRG_95

Name

Cytophotometry
Concept

Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data