Minimum Essential Medium Eagle (MEM) w/ Earle’s salts, L-Glutamine, NEAA, Sodium pyruvate and 15mm HEPES buffer w/o Sodium bicarbonate

• Catalog number
  AT182-20LX1
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• Size
  1X20L

• Description
  The Essential Minimum Medium (MEM) by himedia laboratory is a modification of Basal Medium Eagle (BME). MEM includes a higher concentration of amino acids so as to bring the protein closer together composition of cultured mammalian cells.
• Similar products
• Shelf Life
  Use before the expiry date
• Storage conditions
  stored at 2-8°C
• Appearance
  Off-white to Creamish white, homogenous powder.
• Solubility
  Clear solution at 13.3 gms/L.
• Use
  used in laboratory chemicals, Manufacture of substances
• Technical data
• Data sheet
• Additional description
  Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
• Group
  media

• Gene target
  Minimum   Essential   Eagle   MEM   Earle’s   salts   L-Glutamine   NEAA   Sodium   pyruvate   15mm   w/o   Sodium   bicarbonate  
• Short name
  Minimum Essential Medium Eagle (MEM) w/ Earle’s salts, L-Glutamine, NEAA, Sodium pyruvate 15mm w/o Sodium bicarbonate
• Technique
  Medium, cell culture Media
• Alternative name
  Minimum Essential Medium Eagle (MEM) w/ Earle’s salts, L-Glutamine, NEAA, Sodium pyruvate and 15mm HEPES solution w/o Sodium bicarbonate
• Alternative technique
  cellculture
• Virus
  hhv

MeSH Data

• Name
  In Vitro Techniques
• Concept
  Scope note: Methods to study reactions or processes taking place in an artificial environment outside the living organism.
• Tree numbers
  • E05.481
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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