Anti Rabbit IgG Polyclonal Isotype Control-FITC

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• Catalog number
  BD-RBPLF-100T
• Price:

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• Size
  100 TEST

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• Description
  Anti Rabbit IgG Isotype Control-FITC is a rabbit IgG polyclonal antibody type isotype control, this reagent was conjugated with FITC format and reported for use in flow cytometric analysis as a negative control, it's used for testing the reliabilty of biological tests. Use the same concetration and condition of the specific antibody of interest.
• Other isotypes
• Clone
  POLYCLONAL
• Conjugate
  FITC
• Application
  Flow Cytometry Method
• Formulation
  Phosphate buffred solution, 0,09% sodium Azide, may contain carrier protein-stabilizer
• Expiration
  After 1 year
• Storage
  2-8°C, store in dark, do not freeze
• Remark
  For Recherche Use Only.
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Isotype or positive controls by peptides, antibodies and deactivated samples.
• Properties
  This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by Immunostep. Rabbit antibodies are very stable and can be stored for several days at room temperature. Immunostep adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Group
  isotypes, Polyclonals and antibodies
• Latin name
  Oryctolagus cuniculus

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• Gene target
  -
• Short name
  Anti Rabbit IgG Polyclonal Isotype Control-FITC
• Technique
  Polyclonal, Control, Isotype, Rabbit, anti, FITC, IgG, antibody to, controls, Fluorescein, IgGs, Isotypes and controls, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  IgG, IgG
• Label
  FITC
• Alternative name
  NA
• Alternative technique
  polyclonals, control, controls, rabbit-anti, antibodies, fluorescine, immunoglobulins
• Tissue
  control

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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