HCV Real-TM Quant Dx Real Time PCR Test

PCR for diagnosis

This HCV Real-TM Quant Dx Real Time PCR Test is used as PCR kit for molecular diagnostics with elecrtophoresis, qPCR or real-time PCR of 96 Tests per screening.
Molecular PCR screening kits
ISO CE marked fro IVD

This molecular diagnostic PCR kit is CE approved and complies with the Directive 98/79/EC for IVD, in vitro PCR Diagnostics. The PCR kit is ISO 9001:2008 and ISO 13485:2012, CE marked with the in vitro diagnostic medical divices requirements Annex 2 of list B of the laboratory Directive 98/79/EC and can be used for diagnosis of viruses. The kit inculde all reagents for PCR or RT-PCR of viral DNA or RNA.
PCR tests CE
Gene

Hepatitis C virus (HCV) NS3, NS4, NS genotypes 1, 2, 3, 4, 5, are small (55–65 nm in size), enveloped, positive-sense single-stranded RNA viruses. Hepatitis C virus is the cause of hepatitis C and some cancers such as Liver Cancer (Hepatocellular carcinoma abbreviated HCC) and lymphomas in humans. HCV Core antigens or envelope proteins are used for immunization to obtain diagnostic antibodies.
Group

PCR, polymerase chain reaction
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

Gene target

HCV Real-TM Quant Real Time Test
Gene symbol

MT-TM, TSPAN16, ACP3, P4HTM
Short name

HCV Real-TM Quant Dx Real Time PCR Test
Technique

PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
Alternative name

Hepatitis C virus Real-TM Quant Dx Quantitative real-time PCR test kit Test
Alternative technique

tests, dna-amplification

Identity

HGNC:28858
Gene

P4HTM
Long gene name

prolyl 4-hydroxylase, transmembrane
Synonyms gene name
- prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
Synonyms
- P4H-TM
- PHD4
- PH4
- HIFPH4
- FLJ20262
- EGLN4
- PH-4
Synonyms name
- Prolyl hydroxlase domain-containing 4
- hypoxia inducible factor prolyl 4 hydroxylase
Locus

3p21.31
Discovery year

2008-12-08
Entrez gene record

54681
Pubmed identfication
- 12163023
- 17726031
RefSeq identity
- NM_177938
VEGA ID

OTTHUMG00000074057

Name

Polymerase Chain Reaction
Concept

Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Tree numbers
- E05.393.620.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data