Rabbit Anti-Rat GRP78 Polyclonal: ATTO 390

#

• Catalog number
  SPC-107B-A390
• Price:

  Please ask

  Ask for price
• Size
  200 µl

# #

• Immunogen
  Rat GRP78 (Bip) synthetic peptide conjugated to KLH
• Antibody s target
  Rat GRP78
• Antibody s full description
  Rabbit Anti-Rat GRP78 Polyclonal
• Primary research fields
  Cancer, Heat Shock, Cell Signaling, Trafficking, Chaperones, Organelle Markers
• Antibody s category
  Polyclonal Antibodies
• Antibody s other name
  BIP Antibody, Grp78 Antibody, HSPA5 Antibody, MIF2 Antibody, immunoglobulin heavy chain binding protein Antibody
• Verified applications
  WB, IHC, ICC/IF, IP
• Raised in
  Rabbit
• Antibody s reactivity
  Human, Mouse, Rat, Bovine, Fungi, Chestnut Blight (Cryphonectria parasitica), Hamster, Monkey, Rabbit, African clawed frog (Xenopus laevis), Dog
• Antibody s recommended dilutions for use
  WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Antibody s purified from
  Peptide Affinity Purified
• Recommended buffer for storage
  PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~78kDa.
• Storage recommendations
  -20°C
• Shipping recommendations
  Blue Ice or 4°C
• Certificate of analysis
  A 1:1000 dilution of SPC-107 was sufficient for detection of Grp78 in 10 µg of rat tissue lysate by ECL immunoblot analysis.
• Antibody in cell
  Endoplasmic Reticulum, Endoplasmic Reticulum Lumen, Melanosome, Cytoplasm
• Tissue specificity
  See included datasheet or contact our support service.
• Scientific context
  GRP78 is a ubiquitously expressed, 78-kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
• Bibliography
  1. Cho, S. et al. (2007). Mol Cell Biol 27(1): 368-83. 2. Yang, Y. et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo, S. et al (2006) 26 (15): 5688-97. 4. Yu, Z. et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi, R. et al. (1999) Anticancer Res. 19:4333-4336. 6. Laquerre, S. et al. (1998) J. Virology 72: 4940-4949. 7. Dong, D. et al. (2005) Cancer Res 65(13): 5785-91.
• Released date
  1/Nov/2008
• NCBI number
  NP_037215.1
• Gene number
  25617
• Protein number
  P06761
• PubMed number
  27722180|25124556|25124556|23124854|23372691
• Tested applications
  Immunocytochemistry/Immunofluorescence|Immunoprecipitation|Immunocytochemistry/Immunofluorescence|Western Blot|Western Blot
• Tested reactivity
  Human|Human|Human|Turtle|Mouse
• Antibody s datasheet
  Contact our support service to receive datasheet or other technical documentation.
• Representative figure link
  more | details | see here | read more | look at
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107). Tissue: Hippocampal Section. Species: Mouse. Fixation: 4% Formaldehyde for 12 hours at RT. Paraffin embedded.. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107) at 1:100 for 12 hours at 4°C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Rabbit at 1:250 for 1 hour at RT. Counterstain: Hoechst at 1:1000 for 10 min at RT. Localization: Grp78 staining in mouse pyramidal cell layer.. Magnification: 20x. Slice thickness: 7 microns . Courtesy of: Rachel Reith, NIH/NIMH.. | Western blot analysis of Human, Dog, Mouse Cell line lysates showing detection of GRP78 protein using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107). Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107) at 1:1000. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm . Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm . Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min. | Western blot analysis of Rat Tissue lysates showing detection of GRP78 protein using Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107). Load: 15 µg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody (SPC-107) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. Rabbit Anti-GRP78 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse Hippocampal Section (SPC-107) | Rabbit Anti-GRP78 Antibody used in Western blot (WB) on Human, Dog, Mouse Cell line lysates (SPC-107) | Rabbit Anti-GRP78 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-107) | Rabbit Anti-GRP78 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-107) | Rabbit Anti-GRP78 Antibody used in Western blot (WB) on Rat Tissue lysates (SPC-107)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.2
• Stock availabilit
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Oryctolagus cuniculus, Rattus norvegicus

#

• Gene target
  GRP78   ATTO   390  
• Gene symbol
  HSPA5
• Short name
  Rabbit Anti- GRP78 Polyclonal: ATTO 390
• Technique
  Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit, Rabbits
• Isotype
  Immunoglobulin
• Label
  ATTO 390
• Species
  Rat, Rats
• Alternative name
  production species: rabbit Antibody toRat GRP78 polyclonal: ATTO 390
• Alternative technique
  polyclonals, rabbit-anti, antibodies

Gene info

• Identity
  HGNC:5238
• Gene
  HSPA5
• Long gene name
  heat shock protein family A (Hsp70) member 5
• Synonyms gene
  • GRP78
• Synonyms gene name
  • heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
  • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Synonyms
  • BiP
• Synonyms name
  • glucose-regulated protein, 78kDa
• Locus
  9q33.3
• Discovery year
  1991-07-26
• Entrez gene record
  3309
• Classification
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000020672

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products